"Water Memory" effects to "Digital Biology"...
Do it yourself
We are pleased to inform
you that we
have developed a simple method to demonstrate what has been dubbed "the
memory of water" (we would rather name it now "information of
This method is based on the skin reactivity of rabbits or guinea-pigs.
It implies 3 dilutions, one IV injection and 4 to 6 intradermal
Performing this expt on 4 animals takes more or less 1/2 day of
Aim of the cooperative
As a start, we wish to
with the participation of as many laboratories world-wide as possible, the
existence of biological activity in a solution so diluted that the
of physical molecules is too low to otherwise induce an effect.
Our starting hypothesis
The activity of a substance in
state not only depends on its concentration but also on what appears to
be the "information" of the water molecules in the solution.
The laboratories having
this first experiment will then be provided on their demand with other
protocols demonstrating even more ambitious part of our work, such as
biological activities beyond Avogadro or using digitally recorded
Rationale of the experiment
Bradykinin (BK) or histamine
(H) are known to be ineffective below 0.1 µM. Thus let's
a solution of BK or H at 1pM (1 x 10-12 M), that is, 5 log
the last active dilution. This solution has been prepared in a standard
way, i.e. by dilution with gentle agitation.
We split this 1pM solution
tubes A and B, and vortex the tube B for 15 sec.
Solutions A and B are then
intradermally into guinea-pig or, even better, rabbit skin.
While solution A does not, as
show any activity, solution B initiates a reaction close to that
with 0.1 - 1 µg/ml BK or H.
This would appear as a
indeed incomprehensible according to classical structural biology. Yet
the explanation is simple: at 10-12 M, there are not enough
molecules (6 x 108 molecules/ml) to activate directly the
receptors, yet enough to "inform" water upon agitation.
A strong agitation seems to
the transmission of the molecular signal to perimolecular water. No
of agitation is observed after 10-15 M (600,000
As an image, we could refer to
sound produced by a piano: the resonating box is there to amplify the
emitted by the strings which would otherwise be barely audible.
The perimolecular water
seem to act in about the same way.